Scott Quigley and Louis Dodds did their chances of breaking into the first-team no harm with strong performances for the reserves.
The Valiants defeated Wigan Athletic 4-1 at Vale Park on Tuesday afternoon.
Blackpool loanee Quigley earned a penalty, converted by Ricky Miller, and scored twice in sixty seconds while substitute Dodds created two of the goals and scored the fourth.
Midfielders Manny Oyeleke and Cristian Montano also played in the game as they continued their comeback from injuries.
Port Vale: Hornby, Gibbons, Montano, Pugh, Jackson (Dodds, 45), Hardcastle, Oyeleke, Quigley, Miller, Kanu, Kay