Aziz Deen-Conteh has agreed to leave the club by mutual consent while Sam Johnson and Ryan Lloyd have extended their loan deals.
Deen-Conteh made the first-team squad on one occasion but never made a first-team appearance. Vale have wished him the best of luck in finding a new club.
Meanwhile, goalkeeper Sam Johnson and midfielder Ryan Lloyd have extended their loans at FC Halifax Town and Chester City respectively.
Both players will remain on loan until the end of April.