Chris Neal is awaiting the results of scans after he injured his ankle in the friendly game against Notts County.
Neal was forced off in the opening minutes of the game to be replaced by Sam Johnson.
Manager Rob Page told the media: “It’s always disappointing to see Chrissy come off like that.
“We will know more with the results of the scans, so it’s too soon to speak about that one.”
Vale are well stocked in the goalkeeping department with Sam Johnson and Ryan Boot under contract but the loss of the consistent Neal is still a blow with the keeper one of Vale’s best performers in recent years.