The Valiants will travel to local rivals Walsall in the second round of the Johnstone’s Paint Trophy.
Vale reached the second round after a good 2-0 win over League One Tranmere Rovers.
The full draw is as follows:
Northern Section: Shrewsbury Town v Crewe Alexandra; Rochdale v Bury; Morecambe v Preston North End; Walsall v Port Vale; Doncaster Rovers v Chesterfield; York City v Coventry City; Notts County v Sheffield United; Hartlepool United v Bradford City
Southern Section: Portsmouth v Wycombe Wanderers; Cheltenham Town v Oxford United; Torquay United v Yeovil Town; Plymouth Argyle v Aldershot Town; Northampton Town v Colchester United; Leyton Orient v Barnet; Brentford v Crawley Town; Southend United v Dagenham and Redbridge.
Ties will be played week commencing October 8, 2012.